Thanks for the fascination With this products. Remember to deliver us together with your Get in touch with facts and your local representative will Call you having a tailored quote. Wherever suitable, they might also assist you by using a(n): Believed shipping and delivery time for your personal area Merchandise sample or special present In-lab demonstration By publishing this type, that you are offering your consent to STEMCELL Technologies copyright Inc.
Assess preclinical take a look at compounds and biologics for his or her skills to modulate the immune system.
Flow cytometry may be used to measure the effectiveness of differentiation protocols Using the STEMdiff™ system, by looking at particular marker expression at Just about every phase with the differentiation procedure. It can even be used for other apps, together with cell sorting, immunophenotyping, and purity evaluation.
Get the job done promptly at this action to pool wells into a 15 mL tube. Be aware: For a big-scale dissociation, make use of a multichannel pipette to pool cells into a sterile reagent reservoir. Add the pooled wells right into a fifteen mL tube.
The STEMdiff™ system supplies a standardized technique for differentiating hPSCs into epithelial cells which might be later cultured in 2D or 3D formats with regards to the exploration requires.
Although Now we have circuitously examined nasal epithelial cells in-residence, there are actually publications that report PneumaCult™ supporting this cell type.
There are several commercially available resources for Major airway epithelial cells. Now we have examined HAECs from two vendors, Lonza and Epithelix — both of those have resulted in Stem cell profitable ALI cultures.
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Sure, you’ll discover the techniques to carry out an ICC staining on your own epithelial cells cultured for the ALI During this protocol. Here's an 가슴수술 index of antibodies that could be utilized for the characterization of airway cultures:
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Generally, HSAECs cultured in PneumaCult™-ALI-S Medium will variety a totally differentiated cuboidal epithelium right after 4 to 5 weeks of society. Some donor variability might be expected.
Use cell counts to independent the sample into two tubes – “unstained” and Stem cell “with viability dye”.
Thank you to your curiosity On this merchandise. You should present us with the Speak to data and your local representative will Call you which has a tailored estimate. In which suitable, they can also guide you by using a(n): Approximated delivery time for your personal place Merchandise sample or unique offer In-lab demonstration By publishing this kind, you're providing your consent to STEMCELL Technologies copyright Inc.
The subsequent fluorochrome-conjugated antibodies and dyes are encouraged to aid Investigation of gene-edited CD34+ HSPCs: